p75NTR is involved in the cell death induced by Aβ. A) Cell death quantification with ELISA PLUS Kit. In control cells Aβ induced a 29% of cell death, when we blocked the p75NTR biological activity with prior p75NTR antibody incubation (1:50) during 24 hours, the Aβ did not produce any cell death in choroid plexus. B) Increased levels of caspase-3 and caspase-9 after p75NTR transfection in primary cell cultures. C) Increased of cleaved-caspase-3 in primary culture cells transfected with p75NTR. The choroid plexus culture cells that over-express p75NTR were more sensitive to damage induced by Aβ increasing the cleaved-caspase-3 band in the blot.
Spuch and Carro BMC Neuroscience 2011 12:39 doi:10.1186/1471-2202-12-39