Figure 2.

Proteolytic processing by Amyloid-β (Aβ) in choroid plexus cells. Western blot analysis of homogenates from primary culture cells from choroid plexus treated with Aβ1-40 2.5 μg/mL during 15, 30 and 60 minutes. The samples were prior applied with proteosome inhibitor during 1 hour. The membranes were developed with an antibody directed against the cytoplasmic domain of p75NTR (ICD) donated by B. Carter and M. Chao (9992). The blot revealed fragments at 24 KDa consistent with the p75NTR-CTF, and a fragment at 19 KDa consistent with p75NTR-ICD, also was observed weakly. The treatment with Aβ1-40 induced a release of ICD fragment at 15 and 30 minutes. α-actin was used as a loading control. B) Similar analysis as in A) but in immunofluorescence treated with Aβ1-40 2.5 μg/mL, 60 minutes. C) Similar analysis as in A) but we separated cytoplasm and nuclear fractions. We observed that p75NTR-ICD was accumulated in the nucleus of the cells after Aβ treatment. α-actin was used as a loading control of cytoplasm fraction and CREB was used as a loading control of nuclear fraction. Representative blots are shown

Spuch and Carro BMC Neuroscience 2011 12:39   doi:10.1186/1471-2202-12-39
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