Table 1

Primers used for single-neuron reverse transcription-polymerase chain reaction (RT-PCR)

Gene

Name

Primer Sequences

Sequence Start

Product Length, bp

GenBank Accession Number


PPE

PPE-F

5'-CTACAGGCGCGTTCTTCTCT-3'

273

201

NM_001002927

PPE-R

5'-AGCAGCAAACAGGATGA-3'

561

GAD67

GAD67-F

5'-CAAGTTCTGGCTGATGTGGA-3'

1616

231

NM_008077

GAD67-R

5'-GCCACCCTGTGTAGCTTTTC-3'

1846

5HT3R

5HT3-F

5'-CGTCAAGTCTGGTTTCCTTACC-3'

1794

112

NM_013561

5HT3-R

5'-GCATCTATGCAAGATGATTTGC -3

1905


PPE, preproenkephalin; GAD67, glutamate decarboxylase 67 gene; 5HT3R, 5-hydroxytryptamine (5-HT, serotonin) type 3 receptor gene. Two 5-HT3 receptor subunits were cloned, namely subunit A (5-HT3A) and subunit B (5-HT3B). Although expression of 5-HT3A subunits alone yields functional 5-HT3 receptors, it has been suggested that 5-HT3B subunits modify the physiological and pharmacological properties of 5-HT3 receptors [35,36]. In this study we attempted to detect the 5-HT3A subunits. The primers were used for both the first and the second PCR amplification.

Fukushima et al. BMC Neuroscience 2011 12:36   doi:10.1186/1471-2202-12-36

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