Sciatic nerve ligation increased the number of eGFP-positive neurons. (A) Representative image of eGFP fluorescence in SDH. eGFP fluorescence intensity was higher on the side ipsilateral (the right side of the figure) to sciatic nerve ligation 2 weeks after surgery compared with the contralateral (the left side). (B) Summary of the effect of nerve ligation on the number of eGFP-positive neurons. The number was the total of eGFP-positive neurons in an area of the SDH in an image scanned from a randomly selected section. Open bar indicates the side contralateral to ligation (n = 18); filled bar indicates the ipsilateral side (n = 18). **P < 0.01, significant.
Fukushima et al. BMC Neuroscience 2011 12:36 doi:10.1186/1471-2202-12-36