Effect of forskolin on the number of eGFP-positive neurons. (A, B) Representative images of eGFP fluorescence in the dorsal horn before (A) and after (B) treatment with forskolin. (C) Forskolin (5 μM) was applied as indicated by the horizontal bar. Data points on the graph (open circles and filled circles) indicate percentage of the number of eGFP-positive neurons normalized to the first ones at 10 minutes before forskolin treatment. Open circles and filled circles indicate the mean percentage of the number of eGFP-positive neurons with forskolin treatment (n = 7) and control neurons (n = 7), respectively. Filled squares indicate percent increases in the ratio of the number of eGFP-positive neurons with forskolin treatment to the number of control neurons. *P < 0.05 indicates a significant difference between the forskolin treatment and the control neurons.
Fukushima et al. BMC Neuroscience 2011 12:36 doi:10.1186/1471-2202-12-36