Distribution of eGFP-positive neurons and double-staining IHC colocalization of eGFP expression with endogenous enkephalin. (A) Low magnification of the L4 segment of the spinal cord shows the highest density of eGFP-positive somata and fibers in SDH. (B)-(D) Identification of eGFP-expressing neurons as enkephalinergic by double-staining IHC analysis with anti-eGFP (B, green) and anti-enkephalin (C, red) in SDH of penk1-eGFP BAC transgenic mouse. Scale bar = 200 μm in (A), 20 μm in (B)-(D).
Fukushima et al. BMC Neuroscience 2011 12:36 doi:10.1186/1471-2202-12-36