Taste cell protein expression in dissociated preparations of wild-type and p27-nullizygous CV papillae. (A) Confocal micrographs of small taste cell aggregates from wild-type (+/+) and mutant (-/-) CV papillae that were surgically isolated, enzymatically dispersed onto glass slides and processed for triple-label immunohistochemistry using antibodies to NTPDase2 (red), PLCβ2 (green) and SNAP-25 (magenta). (B) Quantitation of cell labeling for each of the specific proteins, expressed as the percentage of total labeled cells in the preparation. Chi-square test indicates that there is no significant difference between groups with respect to the relative frequency distributions of cells expressing individual proteins. No cell was labeled by more than one antibody in these preparations. (means ± SEM for 3 replicates in each condition; χ2 = 2.348; p = 0.309). Scale bar: 10 μm.
Harrison et al. BMC Neuroscience 2011 12:34 doi:10.1186/1471-2202-12-34