APP processing in WR spinal cord. Western blot analysis of brain stem (BS) and spinal cord (SC) lysates from age-matched WT and WR mice. Total lysates (20 μg per lane) were separated on an 8% polyacrylamide gel, blotted and subjected to immunostaining using 22C11 (APP), LC3 and Rab7 as described. The positions of APP695 and APP770, of LC3I (18kD form) and LC3II (16 kD form) and Rab7 (22 kD) are marked on the left. Anti-tubulin antibody (lower panel) was used as loading control. Band intensities for APP695, LC3II and Rab7 were quantified from 3 independent experiments and plotted as ratio WR/WT ± SEM. For each WT/WR pair, the WT value was set to 1.
Palmisano et al. BMC Neuroscience 2011 12:24 doi:10.1186/1471-2202-12-24