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Endosomal accumulation of APP in wobbler motor neurons reflects impaired vesicle trafficking: Implications for human motor neuron disease

Ralf Palmisano1, Panagiota Golfi1, Peter Heimann2, Christopher Shaw3, Claire Troakes3, Thomas Schmitt-John4* and Jörg W Bartsch15*

Author Affiliations

1 King's College London, Pharmaceutical Science Research Division, 150 Stamford Street, London SE1 9NH, UK

2 Department of Cell Biology, Bielefeld University, 33501 Bielefeld, Germany

3 Kings College London, Institute of Psychiatry, De Crespigny Park, London SE5 8AF, UK

4 Molecular Biology Department, Aarhus University, 8000 Aarhus, Denmark

5 Department of Neurosurgery, University of Marburg, Baldingerstrasse, 35033 Marburg, Germany

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BMC Neuroscience 2011, 12:24  doi:10.1186/1471-2202-12-24

Published: 7 March 2011

Additional files

Additional File 1:

APP/Rab7 as a diagnostic marker for impaired vesicle trafficking in MDF motor neurons. Immunohistochemical staining for Amyloid-Precursor Protein (APP,A) using rabbit anti-APP, and for Rab7 (B) using goat-anti-Rab7, and co-localisation of both proteins (C) in spinal cord motor neurons of 60 d.p.n. MDF mice. Note the large endosomal vesicles (arrows in C) staining positive for APP and Rab7, indicating a transport impairment in these motor neurons, similar to the ones observed in wobbler.

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