Kal7 is expressed in the major neuronal cell types in nucleus accumbens and striatum. Male Tg(Drd1a-EGFP)X60Gsat/Mmmh mice were deeply anesthetized, fixed by perfusion with 4% paraformaldehyde, and brains were sectioned as described . Sections were stained for GFP (green) and Kal7 (red) and imaged with a confocal microscope. A-C. NAc core. D-F. Striatum. Scale bar, 20 μm. A collapsed Z-stack (0.5 μm steps) is shown. A total 249 neurons in six sections from two mice (GFP+Kal7, Kal7 only and GFP only) were counted in the striatum: 98% of the GFP-positive neurons contained Kal7 and 2% did not; 33% of the Kal7-positive neurons did not contain GFP. A total of 239 neurons in six sections from two mice were counted in the NAc: 92% of the GFP-positive neurons contained Kal7 and 8% did not; 31% of the Kal7-positive neurons did not contain GFP.
Mains et al. BMC Neuroscience 2011 12:20 doi:10.1186/1471-2202-12-20