Figure 2.

Principle of STED microscopy. The red depletion beam is phase-modulated to form a focal doughnut shown in the top right panel. Superimposition of this STED focus onto the diffraction limited excitation focus, shown in the adjacent panel, sharpens the effective fluorescence spot, which allows nanoscale imaging. The lower panels show well discernable fluorescent beads (diameter 40 nm) and a line profile across one of them with FWHM-value below 50 nm. Scale bar: 200 nm

Blom et al. BMC Neuroscience 2011 12:16   doi:10.1186/1471-2202-12-16
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