NMDA receptor-dependent LTP is impaired in Kal7KO animals. A) Example traces of evoked fEPSPs before (black) and 60 min after (red) 3 trains of theta burst stimulation (TBS) for a WT and Kal7KO animal. Each TBS train consisted of 10 bursts delivered at 5 Hz, and each burst consisted of 5 stimuli at 100 Hz. Calibration bars: 0.1 mV, 20 ms. Example traces in this and subsequent figures are averages of 10-15 sweeps. B) Group time courses of fEPSP slope recorded from WT (n = 5 animals) and Kal7KO mice (n = 5) following 3 trains of TBS delivered at time zero. C) Group data showing magnitude of LTP measured 60 min post-induction for 1 train of TBS (WT: n = 8; Kal7KO: n = 7), 3 trains of TBS (n = 5 for both WT and Kal7KO), or high frequency stimulation (HFS; 100 Hz/1 sec; n = 5 for both WT and Kal7KO). *, p < 0.05 compared to baseline; #, p < 0.05 between condition. D) LTP induced by TBS or HFS requires NR2B-containing NMDA receptors. Group data showing the lack of TBS-induced or HFS-induced LTP in the presence of the NMDA receptor antagonist CPP (3 μM) in both WT and Kal7KO animals (n = 3-5 slices/condition), and the lack of TBS-induced LTP in WT animals in the presence of the NR2B antagonist ifenprodil (3 μM; n = 5).
Lemtiri-Chlieh et al. BMC Neuroscience 2011 12:126 doi:10.1186/1471-2202-12-126