Detection of HMG-CoA reductase in the cochlea of 5-day-old Wistar rats. Total RNA of the organ of Corti (OC), the spiral ganglion (SG) and the stria vascularis (SV) was reversed transcribed into cDNA and amplified by PCR. Total brain (B) RNA served as a positive control. Omission of cDNA in the PCR mixture served as no template control (NTC). The DNA ladder (M) is indicated with its lengths. β-actin was used to control cDNA synthesis.
Brand et al. BMC Neuroscience 2011 12:114 doi:10.1186/1471-2202-12-114