Table 1

Details on primary antibodies used for immunohistochemistry

Name and product code

Host

Dilution

Detects

Supplier


Calcitonin receptor-like receptor (CLR) 3152

Rabbit

1:500

C-terminal of human CLR

Merck & Co., Inc, USA

CLR 3155

Rabbit

1:500

C-terminal of rat CLR

Merck & Co., Inc, USA

CLR 132

Sheep

1:100

C-terminal of rat CLR

Merck & Co., Inc, USA

Receptor activity-modifying protein 1 (RAMP1) 844

Goat

1:100

C-terminal of human RAMP1

Merck & Co., Inc, USA

RAMP1 3158

Rabbit

1:500

C-terminal of rat RAMP1

Merck & Co., Inc, USA

Calcitonin gene-related peptide (CGRP), polyclonal, B47-1

Rabbit

1:1600

rat CGRP

Europroxima; Arnhem, The Netherlands

CGRP, monoclonal, ab81887

Mouse

1:100

rat α-CGRP

Abcam; UK

Myelin basic protein (MBP) polyclonal, A0623

Rabbit

1:200

Myelin, Schwann cells

Dako; Cophenhagen, Denmark

Synaptophysin, polyclonal, A0010

Rabbit

1:100

Synaptic vesicle protein

Dako; Copenhagen, Denmark

Isolectin IB4 Alexa flour 594 conjugate, I21413

1:50

C-fibers (in general)

Invitrogen; CA, USA


Eftekhari and Edvinsson BMC Neuroscience 2011 12:112   doi:10.1186/1471-2202-12-112

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