CLR/RAMP1 and CGRP double-staining in rat C1. (A) RAMP1 and (B) CLR expression in rat C1. The receptor components are found from Lissauer's tract and within laminae I and II (arrows). Higher magnification discloses expression in the walls of the capillaries. (C) Co-expression of CLR (red) and RAMP1 (green) in the laminae and the capillaries. (D) Double-staining of CGRP (red, thick arrows) and RAMP1 (green, thin arrows) using confocal microscopy. CGRP and RAMP1 are not co-expressed. (E) Fluorescence and DAB staining of the receptor components. Positive fibers are displayed close to the central canal (arrows), where also positive capillaries are found (arrow heads).
Eftekhari and Edvinsson BMC Neuroscience 2011 12:112 doi:10.1186/1471-2202-12-112