Additional file 4.

Application of picrotoxin induces a transient Ca2+-release. Mean (+/- SEM) amplitude of the response (photons/s) versus time of the Ca2+ -induced response (within the ROI) evoked by picrotoxin application (250 μM). This result clearly demonstrates that picrotoxin application induces, per se, a release of Ca2+, which extends for about 10 to 15 min. Note that the amount of released calcium is much lower than the GFP-aequorin capacity to respond to the stimulus. Indeed, the mean of the sum of total photons emitted after picrotoxin application is 11159 for the overall antennal lobes (emitted from all OR83b targeted neurons), while, as an example, GA is able to emit more than 35000 photons just for the few spearmint excited neurons (as compared to Figure 3B1). Finally, remark also that the SEM is larger and more variable after picrotoxin application than before (even difficult to observe at this magnification). (Mean = red line, SEM = blue).

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Murmu et al. BMC Neuroscience 2011 12:105   doi:10.1186/1471-2202-12-105