Table 1

PCR amplification primers

Gene

Primer Sequence

Size (bp)

T(°C)


G3PdH

5'-TGGCATTGTGGAAGGGCTCATGAC-3'

188

60

5'-ATGCCAGTGAGCTTCCCGTTCAGC-3'

NR1 N-ter

5'-AGTCCAGCGTCTGGTTTGAGATGA-3'

235(a)/298(b)

66

5'-CTCGCTTGCAGAAAGGATGATGAC-3'

NR2A

5'-TTCATCTGGGAGCACCTCTT-3'

402

60

5'-GGCCACAAATGTTTGGAGTT-3'

NR2B

5'-GGCATTGCTATCCAAAAGGA-3'

449

60

5'-GACAGGTTGGCCATGTTCTT-3'

NR2C

5'-GCACACCCACATGGTCAAGTTC-3'

327

60

5'-CTGCTCATCACCTCATTCTTCT-3'

NR2D

5'-GCAATACCCACCTCTGAAGTTT-3'

350

60

5'-CCAGCATCTCAATCTCATCGTC-3'

NR3A

5'-GGTTCCCTGCGGAAAGAGAAGC-3'

310/370

60

5'-TACCATGAGCTGCTGGGGTCCC-3'

NR3B

5'-GGTTGGGGACAAAACCTTTGAGG-3'

287

60

5'-CGGTGAGCAGCTTGCAGTCC-3'

NR1 C-ter

5'-ATGCCCCTGCCACCCTCACTTTTG-3'

381(NR1.1)/270(NR1.2)

70

5'-GCAGCTGGCCCTCCTCCCTCTCA-3'

NR1 C-ter

5'-TACAAGCGACACAAGGATGC-3'

241(NR1.3)/132(NR1.4)

60

5'-CGAGGGATCTGAGAGGTTGA-3'


Pregno et al. BMC Neuroscience 2011 12:103   doi:10.1186/1471-2202-12-103

Open Data