Additional file 2.
Figure S2 - The NR2C/D subunit-selective antagonist UBP141 is able to inhibit the NMDA effect on NRG1-induced migration. ErbB4-transfected cells were treated for 18 hrs with different combinations of 8 μM NMDA, 5 nM NRG1, 5 μM UBP141 and 15 μM BAPTA-AM. Notice that UBP141 has no effect per se on ST14A cell migration and that the presence of BAPTA completely masks the inhibitory effect of UBP141 on (NRG1+NMDA)-induced migration. Migration was calculated as percentage of total migrated cells as described in Material and Methods. Biological triplicate experiments were carried out in technical triplicate. Data are presented as means + SD. Statistical analysis was performed by one-way ANOVA followed by Tuckey's post hoc test, significant differences vs control are indicated as * = p < 0.05; ** = p < 0.01; *** = p < 0.001, while significant effects between treatments are indicated as # p < 0.05; ## = p < 0.01; ### = p < 0.001.
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Pregno et al. BMC Neuroscience 2011 12:103 doi:10.1186/1471-2202-12-103