Figure 9.

Conserved effect of dominant negative SCA13 mutation inserted into zebrafish Kv3.3. A) R335H RNA did not generate functional channels when injected alone into oocytes. The membrane was pulsed from a holding potential of -90 mV to voltages ranging from -80 to +60 mV in 10 mV increments. B) RNA for wild type zebrafish Kv3.3 (0.1 ng) and the R335H mutant were mixed in the indicated ratios and injected into oocytes. Representative current traces obtained from each mixture at +40 mV have been superimposed. C) The fraction of wild type current amplitude remaining has been plotted versus the injected ratio of wild type:R335H RNA. Amplitudes were measured at +40 mV. Means differed significantly by one way ANOVA (p < 0.0001) followed by Student's t-test, (1:0, n = 13; 1:0.5, n = 18; 1:1, n = 13; 1:2, n = 15; 0:1, n = 7). Pairwise comparisons: ***, significantly different from wild type alone (1:0), p < 0.001; **, significantly different from 1:1 ratio, p < 0.005.

Mock et al. BMC Neuroscience 2010 11:99   doi:10.1186/1471-2202-11-99
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