P4HB immunostaining for and H&E histological staining of PSI-induced inclusions developed in PC12 cells after proteasome inhibition by PSI. A and A', cells in the vehicle; B-E and B'-E', cells in PSI for 12, 24, 36 and 48 h; F and F', a fraction of inclusions isolated from the cells in PSI for 48 h. Development of inclusions in the cells at the end of indicated points of PSI exposure is indicated as closed arrows, and nuclei and juxta-inclusion nuclei indicated as closed arrow heads. Scale bar, 10 μm.
Li et al. BMC Neuroscience 2010 11:95 doi:10.1186/1471-2202-11-95