Coomassie blue staining of proteins resolved in 2-D gel and identification of P4HB via PMF. A, representative patterns of protein spots: a, cells in vehicle; b, cells in PSI; c or d, the isolated fraction of PSI-induced inclusions before and after protein gel spot picking from 2-D gel. B, identification of P4HB via PMF: a, an expanded full scan of PMF; b, the number of theoretically matched peptides and their sequences after preliminary identification via PMF against the single NCBInr database; c-e, comparable values of Mowse score after consistent identification via PMF against the multiple NCBInr, SwissProt, MSDB databases. Mowse score is -10×Log (P), where P is the probability that the observed match is a random event. Significance levels in the multiple databases are 61 in NCBInr branch, 51 in SwissProt branch and 56 in MSDB branch, respectively (p < 0.05). Close arrows indicate locations of the protein spots of interest in 2-D gel for identification, and theoretical sequences of the matched peptides for P4HB after identification.
Li et al. BMC Neuroscience 2010 11:95 doi:10.1186/1471-2202-11-95