Email updates

Keep up to date with the latest news and content from BMC Neuroscience and BioMed Central.

Open Access Research article

Proteomic characterization of an isolated fraction of synthetic proteasome inhibitor (PSI)-induced inclusions in PC12 cells might offer clues to aggresomes as a cellular defensive response against proteasome inhibition by PSI

Xing'an Li12, Yingjiu Zhang2, Peng Xie3, Jinhua Piao4, Yihong Hu1, Ming Chang1, Tao Liu5 and Linsen Hu1*

Author Affiliations

1 Department of Neurology, The First Affiliated Hospital, Jilin University, Changchun 130021, China

2 Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education (Jilin University), Changchun 130021, China

3 Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

4 Department of Cardiovascular Pediatrics, The First Affiliated Hospital, Jilin University, Changchun 130021, China

5 College of Life Sciences, Jilin University, Changchun 130021, China

For all author emails, please log on.

BMC Neuroscience 2010, 11:95  doi:10.1186/1471-2202-11-95

Published: 12 August 2010

Abstract

Background

Cooperation of constituents of the ubiquitin proteasome system (UPS) with chaperone proteins in degrading proteins mediate a wide range of cellular processes, such as synaptic function and neurotransmission, gene transcription, protein trafficking, mitochondrial function and metabolism, antioxidant defence mechanisms, and apoptotic signal transduction. It is supposed that constituents of the UPS and chaperone proteins are recruited into aggresomes where aberrant and potentially cytotoxic proteins may be sequestered in an inactive form.

Results

To determinate the proteomic pattern of synthetic proteasome inhibitor (PSI)-induced inclusions in PC12 cells after proteasome inhibition by PSI, we analyzed a fraction of PSI-induced inclusions. A proteomic feature of the isolated fraction was characterized by identification of fifty six proteins including twenty previously reported protein components of Lewy bodies, twenty eight newly identified proteins and eight unknown proteins. These proteins, most of which were recognized as a profile of proteins within cellular processes mediated by the UPS, a profile of constituents of the UPS and a profile of chaperone proteins, are classed into at least nine accepted categories. In addition, prolyl-4-hydroxylase beta polypeptide, an endoplasmic reticulum member of the protein disulfide isomerase family, was validated in the developmental process of PSI-induced inclusions in the cells.

Conclusions

It is speculated that proteomic characterization of an isolated fraction of PSI-induced inclusions in PC12 cells might offer clues to appearance of aggresomes serving as a cellular defensive response against proteasome inhibition.