Figure 4.

The effects of cyclic tensile stress on gene expression levels analyzed by real time RT-PCR. Application of cyclic tensile stress resulted in significant increases in mRNA expression levels of platelet derived growth factor receptor (PDGFR), guanine nucleotide binding protein gamma 12 (G12) at 12 hours, neurotrophic tyrosine kinase receptor type 2 (trkA/B), fibroblast growth factor receptor (FGFR), mitogen-activated protein kinase kinase kinase kinase 4 (HGK) at 24 hours, and calcium channel voltage-dependent L type alpha 1F subunit (CACN), v-raf-leukemia viral oncogene 1 (Raf1), dual specificity phosphatase 1 (MKP), mitogen-activated protein kinase 8 interacting protein 3 (JIP3), heat shock protein 72 (HSP72), DNA-damage-inducible alpha (GAD D45), DNA-damage inducible transcript 3 (GAD D153) at 48-72 hours. Red bars indicate significant differences (P < 0.05) in upregulation at least 2-fold in comparison to controls. Data are mean ± SEM of 3 experiments.