Subcellular distribution pattern of exogenous Mayven following drug induced disruption of the OEC cytoskeleton with Cytochalasin B (A-D), Wiskostatin (E-H) and Nocodazole (I-L). Non-transfected (A,B) pMayven-EGFP (C) and pEGFP-N1 (D) transfected cells were exposed to 0.5 μM Cytochalasin B for 36 hrs (B-D) and stained for actin (A-D). Cytochalasin B treatment resulted is a redistribution of Mayven (C) compared to EGFP (D). Transfected pMayven-EGFP cells were administered 2, 4 and 8 μM Wiskostatin for 2 hrs (E-G) and pEGFP-N1 cells were treated for the same duration with 8 μM (H). In the absence of cell death there was a relocation of Mayven (F) while cell death did not accompany any change in the distribution pattern in pEGFP-N1 transfectants (H). Non-transfected cells (I,J) and pMayven-EGFP transfected cells (K,L) were treated with 30 μM Nocodazole for up to 3 hrs (J,L) and immunostained for β-tubulin (I-L). Mayven loclaization remains unaffected despite a breakdown in the microtubular network (K,L).
Montague et al. BMC Neuroscience 2010 11:63 doi:10.1186/1471-2202-11-63