Figure 8.

GPR18 antibody recognizes hGPR18 receptors stably expressed in HEK293-GPR18 cells. Immunofluorescent confocal microscopy was conducted using HEK293 cells stably transfected with HA11-tagged hGPR18 and HA11 (1:500) and GPR18 (1:500) antibodies. a, HA11 antibody (detected with Texas Red secondary; red) staining shows HA11-hGPR18 transfected cells. b, hGPR18 antibody (detected with FITC secondary; green) staining of the same cells identified with the HA11 antibody. c, HA11 antibody (detected with Texas Red secondary; red) staining shows HA11-hGPR18 transfected cells. d, hGPR18 antibody staining (detected with FITC secondary; green) was blocked when the GPR18 antibody was co-incubated with immunizing protein (30 μg/ml) for 1 hour before administration. DAPI (1.5 μg/ml; blue) was used to label the nucleus of all HEK293-GPR18 cells.

McHugh et al. BMC Neuroscience 2010 11:44   doi:10.1186/1471-2202-11-44
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