Confirmed changes in mPFC gene expression. Four changes in gene expression were confirmed in the mPFC with qPCR analysis. (A) Neurofilament light is decreased by 20% at 1-day of abstinence (p < 0.05). (B) CD47 expression is decreased from naïve at 1-day (23%, p < 0.05) and 10-days of abstinence (21%; p < 0.01). (C) Dopamine receptor D5 (Drd5) expression is increased from naïve at 1-day (40%; p < 0.01) and 100-days (41%; p < 0.01). (D) Adenosine A2B receptor (Adora2b) expression is decreased from naïve at 10-days of abstinence (20%; p < 0.05). Statistical analysis was performed by one-way ANOVA with Student-Newman-Keuls post hoc testing; * p < 0.05, ** p < 0.01.
Freeman et al. BMC Neuroscience 2010 11:29 doi:10.1186/1471-2202-11-29