Additional file 1.

The DCX-CreERT2 construct and the generation of DCX-CreERT2 transgenic mouse. (a) Schematic representation of the DCX-CreERT2 construct. The 7.7-kb fragment DNA of Dcx-3'-UTR was cloned by RT-PCR and inserted downstream of the CreERT2. The founders were genotyped by (b) PCR and (c) Southern blot (non-transgenic control DNA in lane "C"). Two weeks after TAM injection, brains of adult mice originating from founder-derived lines mated with Rosa26lacZ mouse (DCX-CreERT2:Rosa26) were further analyzed by X-gal staining. (d) β-gal positive cells in the DG of an adult DCX-CreERT2:Rosa26 mouse two weeks after TAM injection. (e) Absence of β-gal activity in the DG of a DCX-CreERT2:Rosa26 mouse injected two weeks before with the vehicle only (corn oil). Insets show higher magnification of the selected region. E, EcoR V; K, Kpn I. Scale bar in (e) = 50 μm.

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Zhang et al. BMC Neuroscience 2010 11:158   doi:10.1186/1471-2202-11-158