Figure 2.

Post-injury expression of ATF-3. One gene of interest that was examined using PCR was ATF-3. However due to the lack of PCR reaction product in the control animals and presence of PCR reaction product in the experimental animals, calculation of -ΔΔCp values and statistics was problematic. Therefore we probed for expression of ATF-3 protein immunohistochemically. A, C: In uninjured control animals a DTMR labelled LDPT neuron is clearly visible (red) and there is a lack of any ATF-3 labelling (B; green). D, E, F However, when an animal that received a complete T-9 spinal transection was examined 4-days post-injury, the FG labelled LDPT neuron (D; in yellow) colocalizes with the nuclear ATF-3 labelling shown in green (E, F). G, H, I Moreover, when animals that received a T-9 spinal contusion injury are examined 1-week post-injury, the DTMR labelled LDPT neurons (G; in red), again, colocalize with the ATF-3 labelling (H; in green). These findings complement the PCR findings indicating an up-regulation of ATF-3 post-SCI. CNT = Uninjured Control, TXN = Transection, CON = Contusion. Scale Bars = 50 μm

Siebert et al. BMC Neuroscience 2010 11:148   doi:10.1186/1471-2202-11-148
Download authors' original image