Open Access Highly Accessed Research article

Slowing of axonal regeneration is correlated with increased axonal viscosity during aging

Phillip L Lamoureux1, Matthew R O'Toole2, Steven R Heidemann3 and Kyle E Miller1*

Author Affiliations

1 Department of Zoology, Michigan State University, East Lansing, MI 48824-1115, USA

2 Department of Mathematics, Michigan State University, East Lansing, MI, 48824-1115, USA

3 Department of Physiology, Michigan State University, East Lansing, MI 48824-3320, USA

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BMC Neuroscience 2010, 11:140  doi:10.1186/1471-2202-11-140

Published: 25 October 2010

Additional files

Additional file 1:

Movement of the axonal framework occurs during normal axonal elongation of neonatal sensory neurons. This movie demonstrates that mitochondria docked to the axonal framework advance forward during normal axonal elongation. Sensory neurons from neonatal rats were grown on plastic dishes, coated with poly-L-ornithine, for 2-3 days and then were stained with MitoTracker Red CMX-Ros (0.1 µM) on the day of imaging. Fluorescent images were acquired every 2 minutes to visualize mitochondrial distribution and phase images were acquired at 15 to 30 minute intervals to resolve axonal morphology. The time of image acquisition for the phase and fluorescent images is shown as h:min in the movie. The movies were straightened to follow the right axonal branch using the Straighten plugin in ImageJ. A kymograph illustrating the forward advance of mitochondria, stably docked to the axonal framework, is shown on the right hand side of the movie. The kymograph was generated from the straightened image. The length of the arrow above the kymograph represents 1 hour and the vertical scale bar is equal to 20 µm. The dot that moves across the top of the kymograph denotes the time position in the movie. The movie is displayed at 20 frames per second.

Format: MOV Size: 4.2MB Download file

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Open Data

Additional file 2:

Movement of the axonal framework occurs during normal axonal elongation of adult sensory neurons. Sensory neurons from adult rats were grown, labeled, and observed using the same conditions as the neurons from neonatal animals. The time of image acquisition for the phase and fluorescent images is shown as h:min in the movie. The movies were straightened to follow the right axonal branch using the Straighten plugin in ImageJ. A kymograph illustrating the forward advance of mitochondria, stably docked to the axonal framework, is shown on the right hand side of the movie. A kymograph illustrating the forward advance of mitochondria, stably docked to the axonal framework, is shown on the right hand side of the movie. The kymograph was generated from the straightened image. The length of the arrow above the kymograph represents 1 hour and the vertical scale bar is equal to 20 µm. The dot that moves across the top of the kymograph denotes the time position in the movie. The movie is displayed at 13 frames per second.

Format: MOV Size: 1.5MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data