Additional file 11.
Elaboration of PDGFRα on bipotent neuronal/oligodendrocyte precursors (N/OPs) independent of PDGF-AA application following propagation in vitro. N/OPs at 2 h (A-B) in vitro express the bHLH transcription factors, Olig2 and Mash1 (A), in addition to nestin (B). Immunofluorescence microscopic analysis reveals that PDGFRα is not initially expressed by this cellular species in our clonal culture paradigm. However, PDGFRα expression is unequivocally present at 24 h (C-D, arrowheads), demonstrating that N/OPs begin to acquire PDGFRα expression and responsiveness to PDGF-AA, which is required for proliferation and migration of OL progenitors following specification.
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Mercer et al. BMC Neuroscience 2010 11:14 doi:10.1186/1471-2202-11-14