Identification of PKH26-labeled cells with proliferating cell nuclear antigen and doublecortin after transplantation. Colocalization of PKH26-labeled bone marrow cells with proliferating cell nuclear antigen (PCNA) (A-D) and doublecortin (DCX) (E-G) around the infarct penumbra 7 and 14 days following transplantation. PKH26 and PCNA double-labeled cells are small and occur in pair or small cluster (C, D). PKH26 and DCX double-labeled cells have round or oval somata with visible neuronal processes. PKH26 fluorescence appears weaker in these double-labeled cells relative to those seen at 7 days post cell transplantation. Scale bar (in B) = 200 μm for A, B and 50 μm for C-G.
Zhang et al. BMC Neuroscience 2010 11:138 doi:10.1186/1471-2202-11-138