Western analysis showing NF-κB translocation in nucleus from cytoplasm and phosphorylation of IkBα in RGCs facing hypoxic stress. Cell (4 × 105) were cultured in 10 mm culture plates and were treated with 200 μM of CoCl2 for 2 h. After treatment, cytoplasmic and nuclear extracts were prepared for western analysis. Cells treated with CoCl2 showed increased expression of NF-κB in nucleus (A, NF-κB, lane 2) with concomitant decrease in cytoplasmic expression (B, NF-κB, lane 2). These cells showed reduced expression of IkB (C; lane 2) and enhanced expression of its phosphorylated form pIkB in cytoplasm (D; lane 2).
Tulsawani et al. BMC Neuroscience 2010 11:125 doi:10.1186/1471-2202-11-125