The coumarin dyes are fixable. Zebrafish larvae at 6 dpf were stained with BODEC (A) or DIBPBC (B) and fixed in 4% paraformaldehyde. The retinas were sectioned and visualized by confocal laser scanning microscopy. The zebrafish retinas are clearly visualized similar to the in vivo imaging.
Watanabe et al. BMC Neuroscience 2010 11:116 doi:10.1186/1471-2202-11-116