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In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives

Kohei Watanabe16, Yuhei Nishimura1234, Takehiko Oka1, Tsuyoshi Nomoto6, Tetsuo Kon1, Taichi Shintou6, Minoru Hirano1, Yasuhito Shimada1234, Noriko Umemoto1, Junya Kuroyanagi1, Zhipeng Wang1, Zi Zhang1, Norihiro Nishimura25, Takeshi Miyazaki6, Takeshi Imamura6 and Toshio Tanaka1234*

Author Affiliations

1 Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan

2 Mie University Medical Zebrafish Research Center, Tsu, Mie 514-8507, Japan

3 Department of Medical Chemogenomics, Mie University Venture Business Laboratory, Tsu, Mie 514-8507, Japan

4 Department of Bioinformatics, Mie University Life Science Research Center, Tsu, Mie 514-8507, Japan

5 Department of Translational Medicine, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan

6 Corporate R&D Headquarters, Canon Inc., Ohta-ku, Tokyo 146-8501, Japan

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BMC Neuroscience 2010, 11:116  doi:10.1186/1471-2202-11-116

Published: 15 September 2010

Additional files

Additional file 1:

Figure S1: In vivo imaging of the zebrafish retina by combining the coumarin derivatives and transgenic zebrafish expressing GFP in rod photoreceptor cells. Tg (rh:GFP) zebrafish from 1 to 5 dpf were stained with DIBPBC. The retinas were visualized by confocal laser scanning microscopy. The development of rod photoreceptor cells is visualized with high resolution by the counter-staining with DIBPBC.

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Additional file 2:

Figure S2: In vivo imaging of the zebrafish retina by combining the coumarin derivatives and transgenic zebrafish expressing Kaede in retinal ganglion cells and amacrine cells. Tg (huc:Kaede) zebrafish from 1 to 5 dpf were stained with DIBPBC. The retinas were visualized by confocal laser scanning microscopy. The development of retinal ganglion cells and amacrine cells is visualized with high resolution by the counter-staining with DIBPBC.

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