Impact of inhibition of F1, F0-ATP synthase on FCCP-evoked responses of [Ca2+]i in WT and G93A transfected SH-SY5Y cells. A) The quantitative kinetic profile of the oligo and FCCP-evoked [Ca2+]i release in WT transfected SH-SY5Y cells. B) The corresponding quantitative kinetic profile of the oligo and FCCP-evoked [Ca2+]i release in the G93A transfected SH-SY5Y cells. The trace is representative of mean of 4-6 cells in focus stimulated with oligo (5 μg/ml; 6 min) and 2µM FCCP (3 min, normalized data). The horizontal black bars indicate the duration of stimulation by oligo and with FCCP plus oligo. Fura-2 AM signals are represented as F/F0. C) A bar diagram of oligo and FCCP plus oligo-induced Ca2+ release in the WT (N=3, n=20) and G93A (N=3, n=19) transfected SH-SY5Y neuroblastoma cells. Gray bars represent oligo plus FCCP-induced Ca2+ release in WT (F/F0 = 0.2245 ± 0.0727) and G93A (F/F0 = 0.0827 ± 0.0304) transfected SH-SY5Y cells. Striped bars represent oligo-induced Ca2+ release in WT (F/F0 = 0.0454 ± 0.0175) and G93A (F/F0 = 0.0229 ± 0.0161) transfected cells. Values represent means ± SD, *p<0.01, **p<0.001. N= Number of experiments; n= Number of cells.
Jaiswal et al. BMC Neuroscience 2009 10:64 doi:10.1186/1471-2202-10-64