Transduction pattern of the CMV-EGFP lentivirus vector in the DG after delivery to the SR. A: In situ hybridization for EGFP mRNA in the GCL, 1 week after stereotaxic injection of CMV-EGFP to the SR. Inset: higher magnification view of the boxed area. Arrowheads indicate positive EGFP expression in the hilus and SGZ. B: Stereotaxic injection to the SR of a fluorescently labeled transferrin-derived peptide (T12-Cy5, pseudocolored green). NeuN+ cells are shown in red. Inset: higher magnification view of the boxed area. Arrowheads indicate NeuN+ cells in the outer GCL positively transduced with the fluorescent peptide (yellow). Animals were sacrificed 48 h after the injection. C: Higher magnification confocal image showing colocalization (yellow) of T12-Cy5 (red) and NeuN (green) in cells located across the suprapyramydal blade (top) hilus and infrapyramidal blade (bottom) of the DG. The orthogonal projection on the y-z axis shows a gradient of peptide expression from the ML to the H with highest expression in cells located in the outer GCL. D: CMV-EGFP transduction pattern in DIV-5 organotypic hippocampal slice cultures. Inset: higher magnification of the boxed area. The split panels at the bottom show the corresponding EGFP and NeuN signals from the same area. Note the almost complete lack of colocalization. Each image shown is representative of 5 animals independently injected. Scale bars: A, B and D: 100 μm; C: 10 μm. CA1: Cornu Ammonis 1, SR: Stratum Radiatum; ML: Molecular layer; GCL: Granule Cell Layer;H:Hilus; SGZ: Subgranular Zone.
van Hooijdonk et al. BMC Neuroscience 2009 10:2 doi:10.1186/1471-2202-10-2