EGFP+ cell location after injection with CMV-EGFP in the DG. Distribution of EGFP+ cells in the GCL 1 (A) or 5 (B) weeks after stereotaxic injection of CMV-EGFP. The central panels represent the split confocal channels shown merged in left panels. Right panels depict pseudo-colored cell-localization maps, used for quantitative image analyses, generated with Cell Profiler showing the automatically identified EGFP+ and total cells. Scale bars: 20 μm. Each image shown is representative of 5 animals independently injected. C, Schematic diagram depicting the subdivisions of the GCL used for quantitative image analyses, reproduced from , with permission from the authors. Distribution of EGFP+ cells within the GCL 1 (D) or 5 (E) weeks after the stereotaxic injection, normalized to the total number of EGFP+ cells. Each portion of the pie diagrams represents the mean percentage of EGFP+ within internal subdivisions shown in C, indicating the distribution across the GCL, color-coded according to (C).
van Hooijdonk et al. BMC Neuroscience 2009 10:2 doi:10.1186/1471-2202-10-2