Open Access Highly Accessed Research article

Matrix metalloproteinase-7 facilitates immune access to the CNS in experimental autoimmune encephalomyelitis

Lillian A Buhler12, Ramsey Samara13, Esther Guzman1, Carole L Wilson4, Liljana Krizanac-Bengez5, Damir Janigro5 and Douglas W Ethell1*

Author Affiliations

1 Division of Biomedical Sciences, University of California Riverside, 900 University Avenue, Riverside, CA 92521-0121, USA

2 Biochemistry and Molecular Biology Graduate Program, UCR, Riverside, CA 92521, USA

3 Neuroscience Graduate Program, UCR, Riverside, CA 92521, USA

4 Department of Pathology, University of Washington School of Medicine, 300 9th Avenue, Seattle, WA 98104, USA

5 Cerebrovascular Research, Cleveland Clinic Foundation NB20, Neurosurgery, 9500 Euclid Avenue, Cleveland, OH 44195, USA

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BMC Neuroscience 2009, 10:17  doi:10.1186/1471-2202-10-17

Published: 6 March 2009

Additional files

Additional file 1:

Movie from a confocal image stack showing MMP-7 immunopositive cells in a perivascular cuff from a MOG-primed wt mouse, during EAE. The mouse had been vaccinated with MOG 15 days prior and demonstrated a clinical score of 1 at the time of sacrifice.

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Additional file 2:

Confocal microscopy shows co-localization of MMP-7 with tomato lectin and Gr-1. (a) Confocal image of tomato lectin-stained cells (red) in a vascular cuff in the brain of a wt mouse during EAE, 15 days after vaccination with a clinical score of 1. (b) Confocal image of the same section in a showing MMP-7 immunostaining (green). (c) Merged image of boxes a and b shows co-localization of MMP-7 and tomato lectin in a perivascular accumulation of immune cells during EAE. (d) Confocal image of Gr-1 immunopositive cells (red) in the brain of a WT mouse during EAE. (e) Confocal image of the same section in d showing MMP-7 immunopositive cells (green). (f) Merged image of d and e shows co-localization of MMP-7 and Gr-1.

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Additional file 3:

Proliferation responses of splenocytes and lymphocytes isolated from MOG-primed wt and mmp7-/- mice. (a) 3H-Thymidine incorporation of splenocytes isolated from MOG-primed wt and mmp7-/- mice and re-stimulated for 4 days in vitro with 0, 10, 20, or 30 μg/ml MOG. (b) 3H-Thymidine incorporation of lymphocytes from the same mice and re-stimulated for 4 days in vitro with 0, 10, 20 or 30 μg/ml MOG. Although proliferation tended to be lower in splenocytes and lymphocytes isolated from mmp7-/- mice, the differences were not statistically significant in comparison to cells from wt mice.

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