Photomicrographs showing immunofluorescence of synaptophysin in the ventral horn of the lumbar (L3/L4) segment of the spinal cord. Upper panel: a representative example of a cross section of a ventral quadrant of the lumbar segment in an intact rat stained for synaptophysin. Spinal cord laminae are distinguished in L3 segment according to The Rat Brain atlas . Scale bar, 500 μm. Lower panel: representative photomicrographs showing synaptophysin immunofluorescence distribution around large neurons of lamina IX in the lumbar segments of the spinal cord of intact (left), spinal (middle) and spinal trained (right) animals, six weeks after complete spinal cord transection. Note the weaker immunofluorescence around the neuron in the spinal animal than in the intact and spinal trained rat. Scale bar, 10 μm.
Macias et al. BMC Neuroscience 2009 10:144 doi:10.1186/1471-2202-10-144