Images of the spinal cord tissue sections with delineated areas which were subject to quantitative analysis. A. Brightfield photomicrograph showing immunocytochemical labeling of BDNF on a cross section of the lumbar (L3/L4) segment of the spinal cord in the intact rat. Spinal cord laminae distinguished in L4 segment  are overlaid. Areas covering motor nuclei (lamina IX) delineated with dashed black lines were taken for quantitative evaluation of BDNF immunoreactivity. B. Photomicrograph showing immunofluorescence of synaptophysin around a large neuron in the ventral horn of the lumbar (L3/L4) segment of the spinal cord. Delineated areas (white lines) surrounding large neurons of lamina IX were taken for quantitative estimation of the synaptophysin immunofluorescence intensity.
Macias et al. BMC Neuroscience 2009 10:144 doi:10.1186/1471-2202-10-144