Open Access Highly Accessed Research article

Status epilepticus affects the gigantocellular network of the pontine reticular formation

Péter Baracskay13, Viola Kiglics1, Katalin A Kékesi12, Gábor Juhász1 and András Czurkó13*

Author Affiliations

1 Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, H-1117 Budapest, Hungary

2 Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary

3 Institute of Medical Chemistry, University of Szeged, H-6720 Szeged, Hungary

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BMC Neuroscience 2009, 10:133  doi:10.1186/1471-2202-10-133

Published: 13 November 2009

Abstract

Background

The impairment of the pontine reticular formation (PRF) has recently been revealed to be histopathologically connected with focal-cortical seizure induced generalized convulsive status epilepticus. To elucidate whether the impairment of the PRF is a general phenomenon during status epilepticus, the focal-cortical 4-aminopyridine (4-AP) application was compared with other epilepsy models. The presence of "dark" neurons in the PRF was investigated by the sensitive silver method of Gallyas in rats sacrificed at 3 h after focal 4-AP crystal or systemic 4-AP, pilocarpine, or kainic acid application. The behavioral signs of the developing epileptic seizures were scored in all rats. The EEG activity was recorded in eight rats.

Results

Regardless of the initiating drug or method of administration, "dark" neurons were consistently found in the PRF of animals entered the later phases of status epilepticus. EEG recordings demonstrated the presence of slow oscillations (1.5-2.5 Hz) simultaneously with the appearance of giant "dark" neurons in the PRF.

Conclusion

We argue that the observed slow oscillation corresponds to the late periodic epileptiform discharge phase of status epilepticus, and that the PRF may be involved in the progression of status epilepticus.