Transfection of pEGFP-N1-hPGRN plasmid shows granular morphology and particular PGRN localization in Golgi. The overlap seen with PGRN and the Golgi marker GM130 (a) is very prominent, while there is no overlap between mitochondria (b) and PGRN. Panel (c) reveals the pEGFP-N1 vector only transfection, having primarily nuclear GFP signal, and a very different subcellular distribution of PGRN, which appears distinctly granular in panels (a and b). Confocal images were taken at 63×.
Ryan et al. BMC Neuroscience 2009 10:130 doi:10.1186/1471-2202-10-130