Subcellular localization of PGRN within motor neurons in primary cultures relative to a marker for the lysosomal compartment markers for neurotransmitter vesicle trafficking and release (SNAP-25 and synaptophysin). Immunolabelling of motor neurons in dissociated spinal cord-DRG cultures with LysoTracker™ (a), SNAP-25 (b), Synaptophysin (c), and anti-PGRN (middle column). Confocal images were captured at 63× magnification, hatched boxes represent 3-5× zoom.
Ryan et al. BMC Neuroscience 2009 10:130 doi:10.1186/1471-2202-10-130