PGRN knockdown reduces cell proliferation and exogenous PGRN rescues cell proliferation. (A) AlamarBlue cell proliferation assay allowing the quantitative measurement of cell proliferation from day 0 to day7 in the presence of serum in 96 well plates. PGRN knockdown significantly reduced the cell proliferation rate from day 2-3 to day 7-8 (10 replicates per determination, p > 0.001). (B) Addition of exogenous PGRN rescued NSC-34 cell proliferation from day 2-3 to 4-5 (representative experiment, 10 replicated per determination, P < 0.05). (C) Apoptosis assay based on TUNEL labelling method following 3 days in the presence of serum. PGRN silencing had no significant effect on apoptosis (N = 2, 6 fields 10× magnification per determination).
Ryan et al. BMC Neuroscience 2009 10:130 doi:10.1186/1471-2202-10-130