PGRN is a sufficient trophic stimulus to maintain prolonged survival of NSC-34 cells in serum-free medium. NSC-34 cells stably transfected with pcDNA3/PGRN and grown in serum-free medium (A) for 20 days in six well plates, the cut-out box illustrating the same cells photographed 3 hr later showing continued active extension and retraction of processes; (B) for 51 days, and (C, D) for 67 days in serum free medium. All NSC-34/vector control cells died before day 20 (not shown). Images were taken at an original magnification of 15×.
Ryan et al. BMC Neuroscience 2009 10:130 doi:10.1186/1471-2202-10-130