Additional file 7.Schematic of experimental paradigm used to count cells in laminin-treated monolayer cultures. Phase contrast (a) and GFAP immunoreactivity (b) of representative cultures plated under glial differentiating conditions on laminin. Five fields (shaded areas) per n = 5–10 cultures were obtained for quantification. Fields of interest were located 30 μm (white line) from the identified core of the neurosphere (black line). Format: TIFF Size: 1.9MB Download file Imbeault et al. BMC Neuroscience 2009 10:13 doi:10.1186/1471-2202-10-13 |