Additional file 2.
Cx30 is expressed by Type 1 and 2a NPCs. (a) Hippocampal NPCs cultured as neurospheres were dissociated on DIV 14 and triple-labelled for Cx30, nestin, and GFAP. Two and three dimensional histograms from a representative flow cytometry analysis are presented for both isotype control (left panel) and experimental (right panel) reactions. The quadrant percentages represent the percentage of total cells expressing corresponding cell markers. (b) Quantitative analysis of Cx30 expression in Type 1 (GFAP+/nestin+) NPCs, Type 2a (GFAP-/nestin+) NPCs, and committed astrocytes (GFAP+/nestin-). The percentage of cells co-expressing Cx30 was calculated divided by the total percentage of each cell type by the total percentage of cells within each cell type expressing Cx30 in triple-labelling studies. Data are presented as mean ± SEM and represent the average of three independent flow cytometric analyses/cultures.
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Imbeault et al. BMC Neuroscience 2009 10:13 doi:10.1186/1471-2202-10-13