Additional file 1.
Immunocytochemical detection of differentiation into mature astrocytes or neurons. F3 (A, C) or F3.Olig2 (B, D) cells were grown on coverslips in DMEM with 2% FBS for 5 days and then fixed with 4% paraformaldehyde. Then the cells were stained with anti-GFAP (A, B) or anti-MAP2 (C, D) antibodies. Some of F3 cells showed differentiation into astrocyte or neurons, but virtually no F3.Olig2 cells were immunoreactive against GFAP or MAP2.
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Hwang et al. BMC Neuroscience 2009 10:117 doi:10.1186/1471-2202-10-117