Additional file 1.

Immunocytochemical detection of differentiation into mature astrocytes or neurons. F3 (A, C) or F3.Olig2 (B, D) cells were grown on coverslips in DMEM with 2% FBS for 5 days and then fixed with 4% paraformaldehyde. Then the cells were stained with anti-GFAP (A, B) or anti-MAP2 (C, D) antibodies. Some of F3 cells showed differentiation into astrocyte or neurons, but virtually no F3.Olig2 cells were immunoreactive against GFAP or MAP2.

Format: TIFF Size: 3.6MB Download file

Hwang et al. BMC Neuroscience 2009 10:117   doi:10.1186/1471-2202-10-117