Measurement of myelin thickness in the spared white matter. (A-C) Representative images of the ventral white matter in toluidine-blue stained semithin sections from (A) Vehicle, (B) F3, and (C) F3.Olig2 groups. Asterisks indicate degenerating axons. Scale bar = 10 μm. (D) Comparison of the mean myelin ratio. N = 3, 6, and 7 animals for Vehicle, F3, and F3.Olig2 groups, respectively. Error bars represent mean ± SD. * = p < 0.05, *** = p < 0.001, by one-way ANOVA followed by Tukey's posthoc analysis. (E) Cumulative frequency histogram of the myelin ratio. The number of axons analyzed was 692, 1311, and 1348 in Vehicle, F3, and F3.Olig2 groups, respectively.
Hwang et al. BMC Neuroscience 2009 10:117 doi:10.1186/1471-2202-10-117