Transplantation of Olig2 overexpressing human NSCs after contusive spinal cord injury. (A-D) Immunofluorescence staining of the transverse sections close to the epicenter at 2 weeks after transplantation of F3 (A, B) and F3.Olig2 (C, D) cells. Grafted human NSCs were identified by immunoreactivity against human specific mitochondria (red). GFAP staining (green) was performed to depict the appearance of spinal cord lesions. Photomicrographs in B and D are magnified images of the boxed regions in A and C, respectively. (E, F) Bright field microscopic images of the transverse sections located at 2 mm rostral to the epicenter at 7 weeks after transplantation of F3 (E) and F3.Olig2 (F) NSCs. Many F3.Olig2 cells were observed in the white matter at this time point. Dotted lines indicate boundaries of the gray matter. Scale bar = 500 μm. (G) The number of human mitochondria positive cells was stereologically counted in the spinal cord sections from the epicenter to 4 mm rostral and caudal regions at 1 mm interval. (H) The percentage grafted cells in the ventrolateral white matter versus total number of grafted cells in the entire transverse sections. * = p < 0.05, ** = p < 0.01, and *** = p < 0.001 by unpaired T test at each distance from the epicenter. E represents epicenter. Error bars indicate mean ± SEM. N = 4 animals per group.
Hwang et al. BMC Neuroscience 2009 10:117 doi:10.1186/1471-2202-10-117